Nature ProtocolsNature Protocols publishes secondary research articles - predominantly Protocols and techniques-related Reviews, Perspectives, Correspondences, Matters Arising, Commentaries, and Consensus Statements - all of which are based on published primary research papers. It covers new techniques and technologies, as well as established methods, used in all fields of the biological, chemical and clinical sciences. Each Protocol contains a full list of reagents and equipment, timing information, and step-by-step procedures (instructions for performing the experiment), as well as information on designing and adapting the technique, its advantages and limitations compared to alternatives, troubleshooting, analysing data, and interpreting results.